Journal: Oncogene

Article Title: Androgen regulation of soluble guanylyl cyclasealpha1 mediates prostate cancer cell proliferation.

doi: 10.1038/sj.onc.1209956

Figure Lengend Snippet: Figure 6 sGCa1 is overexpressed in advanced prostate cancer tissues. (a) Total mRNA was isolated from prostate tissues (acquired from CHTN), which are normal (N1), BPH (B1–B3), or MPC (C1, C2) and subjected to semi-quantitative RT-PCR to measure the expression of sGCa1, sGCb1, PSA, EZH2, E-cadherin, and AR. (b) The human prostate cancer tissue array slide (from Imgenex) was prepared and subjected to immunohistochemistry analysis to detect the expression of sGCa1 protein. Staining results from one or more representative tissues, of different stages, are shown. DAPI staining shows cell nuclei in the lower panels. (c) The sGCa1 expression levels of 45 tissue samples (five normal; eight stages 1 and 2; 32 stages 3 and 4) were quantified according to manufacturer’s protocol (Imgenex). Note that sGCa1 expression levels are represented relative to the average expression level of normal tissues, which was set to 1. Data represent mean expression values plus/minus s.d.

Article Snippet: Prostate tissue array slide (Imgenex) was processed according to the manufacturer’s protocol.

Techniques: Isolation, Quantitative RT-PCR, Expressing, Immunohistochemistry, Staining

Journal: International journal of cancer

Article Title: Increased expression of prostate-specific G-protein-coupled receptor in human prostate intraepithelial neoplasia and prostate cancers.

doi: 10.1002/ijc.20635

Figure Lengend Snippet: FIGURE 1 – Specific expression of PSGR in human prostate tissues using in situ hybridization. (A) Overexpression of PSGR in human prostate malignant tissues with different clinical stages and Gleason scores. (A-a) Gleason score 7, clinical stage II, T2N0M0. (A-b) Gleason score 4, clinical stage III, T3N0M0. (A-c) Gleason score 9, clinical stage IV, T4NXM1. (A-d) Gleason score 9, clinical stage IV, T3N1M0. (B) PSGR expression in prostate PIN and normal tissue. Strong PSGR expression is noted in varied stages of PIN (arrow) samples, whereas very low amount of PSGR transcripts are evident in adjacent normal prostate tissues (N, arrow). (B-a) Expression of PSGR in tufting pattern of PIN and normal prostate tissue. (B-b) PSGR expression in the micropapillary pattern of PIN lesion. (B-c and B-d) In situ hybridization of PSGR expression using PSGR sense probe as a control. (C) Expression of PSGR in nonprostate malignant tissues. No PSGR expression was detected in other malignant tissues. (C-a) Breast cancer tissue. (C-b) Lung cancer. (C-c) Stomach cancer. (C-d) Uterine cancer tissue.

Article Snippet: In situ hybridization of PSGR in prostate tissues Tissue slides containing 56 prostate tumors and 11 nonprostate tumors of varied clinical stages, 6 normal prostate tissues and 11 normal nonprostate tissues embedded in paraffin were bought from IMGENEX.

Techniques: Expressing, In Situ Hybridization, Over Expression, Control

Journal: International journal of cancer

Article Title: Increased expression of prostate-specific G-protein-coupled receptor in human prostate intraepithelial neoplasia and prostate cancers.

doi: 10.1002/ijc.20635

Figure Lengend Snippet: FIGURE 2 – The expression of PSGR mRNA level (copies/103 -ac- tin) in normal prostate tissue (normal, 19), BPH (8) and PCa (57) determined by real-time RT-PCR method. The expression level of PSGR mRNA detected in the prostate tumor tissue is significantly higher than that from the normal prostate tissue and BPH tissue (p 0.001, Mann-Whitney U-test). No significant difference was detected for the expression of PSGR between normal prostate and BPH (p 0.05, Mann-Whitney U-test).

Article Snippet: In situ hybridization of PSGR in prostate tissues Tissue slides containing 56 prostate tumors and 11 nonprostate tumors of varied clinical stages, 6 normal prostate tissues and 11 normal nonprostate tissues embedded in paraffin were bought from IMGENEX.

Techniques: Expressing, Quantitative RT-PCR, MANN-WHITNEY

Journal: International journal of cancer

Article Title: Increased expression of prostate-specific G-protein-coupled receptor in human prostate intraepithelial neoplasia and prostate cancers.

doi: 10.1002/ijc.20635

Figure Lengend Snippet: FIGURE 3 – ROC for PSGR real-time PCR assay. The assay has high-level sensitivity and specificity for discriminating benign prostate tissue from malignant prostate tissue in laboratory (AUC 0.902 0.032).

Article Snippet: In situ hybridization of PSGR in prostate tissues Tissue slides containing 56 prostate tumors and 11 nonprostate tumors of varied clinical stages, 6 normal prostate tissues and 11 normal nonprostate tissues embedded in paraffin were bought from IMGENEX.

Techniques: Real-time Polymerase Chain Reaction

Journal: bioRxiv

Article Title: PSMA Expression in the Hi-Myc Model; Extended Utility of a Representative Model of Prostate Adenocarcinoma for Biological Insight and as a Drug Discovery Tool

doi: 10.1101/439596

Figure Lengend Snippet: Immunohistochemistry using anti-PSMA antibodies in wild type mouse kidney (A) shows high expression in proximal tubules. Negative staining in the kidney of a Folh1 knockout mouse (B) confirms antibody specificity. Normal mouse prostate (C) shows no staining. Human prostate cancer (D) shows high PSMA expression concentrated at cell membranes. Hi-Myc PIN (E) and adenocarcinoma (F) show high PSMA expression with a similar pattern. Western blot (G) shows similar results with expected expression in kidney, brain, and salivary gland (SG), but not in wild type prostate anterior lobe (AP) or lateral lobe (LP). Hi-Myc prostates show PSMA expression proportional to the expected tumor burden in AP (low burden) and LP (high burden).

Article Snippet: Slides were incubated with antibodies directed against PSMA (Cell Signaling Technology rabbit monoclonal antibody (D7I8E).

Techniques: Immunohistochemistry, Expressing, Negative Staining, Knock-Out, Staining, Western Blot